Olympus VS120 Virtual Slide Microscope (VS120-S6-W)   Room#:1024

Introduction:

The VS120 not only creates high resolution brightfield images, but also can scan in full multi-fluorescence mode (4 kind wavelength of fluorescence). Scanning multiple large specimens up to 31 Z-planes that enables it to be a powerful tool for the viewing of thicker specimens, such as cell clusters or cranial nerves. An innovative new algorithm makes image stitching more precise than ever, enabling high-level accuracy that can be applied from small animal brain slices to large specimens. Thus, the VS120 switches seamlessly between micro and macro observation to enable swift viewing of regions of interest and overall structures alike.

Application:

This system is mainly used to scan specimen on glass slides, such as brain slices and other tissue slices. The efficiency of scanning is greatly improved by the 6 slides motorized stage. At the same time, it is equipped with the stage that can hold two 2 "×3" slides which is suitable to observe larger specimens.

Fluorescence Observation: 

Cy5: Ex. 628/40 nm; Em. 692/40 nm

TEXRED: Ex. 586/20 nm; Em. 628/32 nm

Cy3: Ex.540/25 nm; Em.605/55 nm (新增)

GFP: Ex. 472/30 nm; Em. 520/35 nm

DAPI: Ex. 365/10 nm; Em. 440/40 nm

    The list of the most suitable dyes:

        

Channels	Dyes
DAPI Ex. 365/10 nm; Em. 440/20 nm	DAPI(359/461)   LysoSensor Blue(374/424)   Marina Blue(363/461)   Vybrant DyeCycle Violet(369/437)
GFP Ex. 472/30 nm; Em. 520/35 nm	5-FAM (5-carboxyfluorescein)(492/518)   CFP2(490/510)   Cy2™(492/507)   DiO(475/500)   ecliptic pHluorin pH5.5(473/507)   Emerald(491/511)   FITC (Fluorescein)(495/519)   GFP (EGFP)(489/511)   MitoTracker™ Green(490/512)   NBD-X (MeOH)(467/538)   PKH67(488/500)   sgGFP™ (super glow GFP)(472/506)   SYTO 16(489/520)   SYTO 9(483/500)   TurboGFP(482/503)   wtGFP (wild type GFP, non-UV excitation)(474/509)   YO-PRO-1(491/506)   YOYO-1(491/508)
TEXRED 562/40 nm; Em. 624/35 nm	5-ROX (carboxy-X-rhodamine)(578/604)   Alexa Fluor® 568(579/603)   Alexa Fluor® 594(590/618)   ATTO 590(594/624)   BOBO™-3(570/605)   Calcium Crimson(589/609)   CellTracker Red CMTPX(586/614)   DyLight 594(593/618)   FluoSpheres Red fluorescent microspheres(577/607)   HCS LipidTOX Red neutral lipid stain(582/616)   HCS LipidTOX Red phospholipidosis(584/608)   HiLyte Fluor™ 594(592/616)   KFP-Red(575/599)   mCherry(587/610)   MitoTracker™ Red(578/598)   ReAsH-CCPGCC(592/606)   Red 580(580/603)   SNARF (carboxy) 514 Excitation pH9(576/638)   SNARF-1 488nm (ph 9.0)(576/638)   SNARF-1 514nm (ph 9.0)(576/638)   Texas Red dextran(592/613)   Texas Red DHPE(584/608)   Texas Red-X® (595/613)   TurboFP635(Katushka)(591/638)   X-Rhod-1 Indicator(580/601)
CY5   Ex. 628/40 nm; Em. 692/40 nm	ATTO 647(644/670)   ATTO 647N(645/668)’   Cy5™(649/666)   DiD (DiIC18(5)) - Lipophilic Tracer(648/670)   DyLight 649(651/673)   HiLyte Fluor™ 647(649/673)

Observable specimen: Glass slide with cover glass

Scan area: Slide glass size 26 mm x 76 mm

Scan time:Approx. 2 min. (20x objective, scan area 15 mm x 15 mm brightfield)

Scan resolution: 

10x (NA 0.4): 0.66μm/ pixel 

20x (NA 0.75): 0.33 μm/pixel 

40x (NA 0.95): 0.17 μm/pixel 

Objectives:2x,10x, 20x, 40x

Size of cover glass: 0.12mm–0.17mm

Objective stage:6 slides motorized stage, two 2″×3″slides stage