Instrument name:Opera PhenixHigh throughput and high connotation imaging analysis system 

Room number：1020 

Instrument introduction 

Opera phenixThe screening system of high-throughput and high connotation imaging analysis system is a new generation of imaging system based on Nipkow dual turntable scanning laser confocal. The dual turntable structure can support synchronous multi-color confocal image acquisition, minimize spectral cross interference, improve imaging speed and sensitivity, and reduce the effects of phototoxicity and photobleaching on living cell observation, and improve light efficiency. The system is equipped with a water mirror and a full-automatic objective replenishing water circulation system. The water mirror has high light efficiency, requires shorter exposure time, is more suitable for long-term living cell detection and can achieve high imaging quality in thick samples. DPC imaging mode can obtain more abundant cell biology information. 

Scope of application 

The system can provide a variety of solutions for automatic, high-speed and high-resolution imaging screening, and can meet the needs of drug discovery and high-throughput biology. It is suitable for cell screening at subcellular resolution level and various screening applications based on micro beads, including the range from conventional micro porous plate to micro nano plate. Two channel simultaneous imaging without crosstalk can be used for FRET experiment。 

Key technical indicators： 

Light source: built in 4-color solid-state laser:405nm，488nm，561nm，640nm Near infrared LED lighting as bright field light source 

Imaging mode: wide field fluorescence imaging, confocal imaging, bright field imaging, label free digital fluorescence (DPC) imaging; 

 Channels：DAPI: EX：405nm;  EM: 435-480nm;

                 GFP: EX： 488nm;  EM: 500-550nm;

                 CY3: EX： 561nm;  EM: 570-630nm;

                 CY5: EX： 640nm;  EM: 650-760nm;      

Detector: built in high-sensitivity and high-resolution synchronous detector (dual camera SCMOS, 16 bit), which can obtain fluorescence and bright field images of different wavelengths at the same time, with the maximum daily imaging flux not less than 100000 pieces; 

Objective lense:

1.25x (Air, NA=0.03,WD=4)

5x (Air, NA=0.16, WD=12.1)

10x (Air, NA=0.3, WD=5.2)

20x (Air, NA=0.4, WD=8.28, Correction collar)

20x (Air, hNA=0.8, WD=0.55)

20x (Water, NA=0.8, WD=0.55)

40x (Air, NA=0.6, WD=3.28, Correction collar)

40x (Water, NA=1.1, WD=0.62)

63x (Water, NA=1.15, WD=0.6) 

Electric scanning stage: high-precision magnetic suspension coding electric XY stage (step accuracy of 50nm, repetition accuracy of 100nm) 

Adapter: 96 hole plate adapter; slide adapter (4 pieces)； 

Environmental control unit:

Temperature control: 37-42 (± 1) degrees Celsius； 

CO2 concentration adjustable：1-10%（± 0.5%）

Image processing software: harmony acquisition and full function module analysis software, including 25 analysis modules and 37 preset application analysis solutions; with texture analysis function, 8 texture filters; with machine self-learning function; with star analysis function, 207 morphological parameters; with data clustering analysis function; with unmarked cell analysis DPC module; with cell three-dimensional view Measurement module, built-in 3D co location analysis function.