Lavision Biotech Light Sheet Microscope Ultramicroscope II   Room#: 1023

Introduction

The bidirectional triple light sheet technology of the UltraMicroscope II generates 6 focused light sheets to excite samples from the side while the fluorescence light is detected by a sCMOS camera perpendicular to the illumination plane. Moving the sample through the light sheet generates a 3D image stack. The dynamic horizontal light sheet focus guarantees excellent Z-resolution covering the entire field of view. Selective excitation of the focal plane reduces bleaching and photo toxicity significantly.

Application:

Together with the multi refractive index compensation, the chromatic correction from 400 nm to 800 nm, and the large and open sample chamber, UltraMicroscope II can be adapted to different samples and clearing protocols.

Technical indicators:

Laser: 405nm、488nm、561nm、639nm.

Light sheet: Bidirectional sheet excitation allows a consistent illumination; minimum thickness＜4.5 um.

Detector: sCMOS, pixel count 2048×2048，pixel area 6.5×6.5um3，sensor area 13.3×13.3mm2, quantum detection efficiency reaches more than 75% at 500nm).

Objectives: 1.1x (NA=0.1, work distance 17.6 mm);

4x (NA=0.30, work distance 5.6-6.0mm);

12x (NA=0.53, work distance 9.0mm).

Sample uptake: up to 30mm×30mm×15mm.

Scan zoom: 2x.