Instrument Name:

Lavision Biotec Light Sheet Microscope (Ultramicroscope II)  Room: X210

Introduction

The bidirectional triple light sheet technology of the UltraMicroscope II generates 6 focused light sheets to excite samples from the side while the fluorescence light is detected by a sCMOS camera perpendicular to the illumination plane. Moving the sample through the light sheet generates a 3D image stack. The dynamic horizontal light sheet focus guarantees excellent Z-resolution covering the entire field of view. Selective excitation of the focal plane reduces bleaching and photo toxicity significantly.

Application

Together with the multi refractive index compensation, the chromatic correction from 400 nm to 800 nm, and the large and open sample chamber, UltraMicroscope II can be adapted to different samples and clearing protocols.

Key Technical Specifications

Laser:

405nm, 488nm, 561nm, 639nm

Light Sheet:

Bidirectional triple light sheet for dual-side excitation; minimum thickness < 4.5 µm

Detector:

sCMOS, 2048×2048 pixels, pixel size 6.5×6.5 µm, sensor area 13.3×13.3 mm², quantum detection efficiency ≥ 75% at 500 nm

Objectives:

1.1x (NA=0.1, WD 17.6 mm); 4x (NA=0.30, WD 5.6-6.0 mm); 12x (NA=0.53, WD 9.0 mm)

Sample Volume:

up to 30 mm × 30 mm × 15 mm

Scan Zoom:

2x