Equipment: Leica two-photon upright microscope (Stellaris 8 DIVE)

Room：1018

     Description：Combining the technology of laser scanning confocal microscopy and two-photon excitation technology, Stellaris 8 DIVE can penetrate deep inside the tissue and excitation of fluorescent molecules. The long wavelength can reduce the damage of the laser on the organism, reduce phototoxicity, as well as ensure high spatial resolution (imaging depth can exceed 1mm) at the same time. It is equipped with a 4TUNE spectral detector, which can freely adjust the detection band in the range of 380-800nm, and combined with an adjustable spectral laser, it can perform multi-color imaging of samples. The detector has a photon counting function, combined with a pulsed laser can be fluorescence lifetime imaging of the sample, equipped with a TauSense module can be split by lifetime information can be added to the sample another dimension of information. The device can also be used alone with single photon imaging capabilities, which are essentially the same as the platform Leica SP8.

      Scope of application: The system's higher imaging depth and low phototoxicity are suitable for long-time real-time imaging of live animals; the principle feature that two-photon can only excite the focal plane signal allows for more accurate photoactivation, photobleaching and other light stimulation experiments; its free spectral adjustment and adjustable wavelength laser are equipped for imaging of multiple standard samples. Equipped with TauSens module, combined with the fluorescence lifetime characteristics of the dye, fluorescence lifetime imaging can be performed.

     Key technical specifications：

                      Light source: Continuous Wave Laser: 405nm、488nm、561nm、638nm

                         Femtosecond Pulse Laser: 1045nm, 680-1300nm wavelength adjustable

                     Imaging modes: wide-field fluorescence imaging, bright-field imaging, single-photon confocal imaging, two-photon imaging

                      Detectors: built-in detector (single photon): 3 spectral scan HyD S

                      External detector (two-photon): 2 spectral scanning HyD NDD (ultra-sensitive detector), 1 spectral scanning PMT (photomultiplier tube)

                     Objective: HC PL APO 10x (Air, NA=0.4, WD=2.8mm)

                                      HC PL APO 20x (Air, NA=0.75, WD=0.62mm)

                                      HC IRAPO L 25x (Water, NA=1.0, motCORR, WD=2.6mm)

                                      HC PL IRAPO 40x (Water, NA=1.1, CORR, WD=0.65mm，salt water immersion available)

                                      HC PL APO 63x (Oil CS2, NA=1.4, WD=0.14mm)

                     Carrier table: motorized carrier table, moving accuracy 0.1um

                     Scanning resolution: 4×1 to 8192×8192. 8192×8192 resolution and 16-bit grayscale depth can be achieved for each channel.

                 Scanning mode: high-resolution (Galvano-mirror) and high-speed scanning mode (Resonant-scanner) can be selected, high-    resolution mode scanning speed 10fps@512×512, scanning field of view 22mm. high-speed scanning mode scanning speed 28fps@512×512.

                     Scanning zoom: zoom range 0.75x ~48x, continuous zoom.

                      Image processing software: LAS X, including 3D visualization, intracellular structure measurement, serial color separation, full specimen navigation and puzzle