Olympus One Laser Two-Photon Upright Microscopy (FVMPE-RS)

Room: B311 

Introduction

       The Olympus FVMPE-RS satisfies a myriad of performance needs for deep observation. Femtosecond lasers can penetrate deep into tissues to excite fluorescent molecules with little phototoxicity to organisms. The two-photon has a Deep Focus Mode, which can achieve deeper imaging than the traditional mode. FVMPE-RS can support long-term live imaging while ensuring high spatial resolution (imaging depth can exceed 1mm). Due to the unique four-axis automatic correction technique which adjust the laser angle and position automatically, FVMPE-RS achieve multi-color laser two spots completely coincide when adjusting any laser wavelength. ANALOG transducers synchronize external devices such as electrophysiology with functions such as two-photon imaging and laser stimulation. Through input or output signals trigger electrophysiology and other external experimental equipment, which support TTL, I/O signals. The Analog unit also uses software to convert voltage into images, and can do the same for fluorescent images, synchronizing the electrical signal measured with the patch-clamp during light stimulation with the captured image and displaying it as a pseudo-color of light intensity. The image workstation and analysis software can control all hardware devices, including functions such as Analog external trigger signal control. As for the mass data in the later stage of imaging results, it can perform three-dimensional reconstruction, surface rendering, magnification and rotation display, and other image functions.

Application:

       The system has the characteristics of high imaging depth and low phototoxicity, which is suitable for long-term real-time imaging of live animals. Two photons can stimulate the focal plane signal only, which is more suitable for accurate light activation, photo bleaching and other light stimulation experiments.

Technical indicators:

Laser: Single line IR laser: 800-1300nm wavelength tunable laser

       Imaging modes: widefield fluorescence imaging, brightfield imaging, two-photon imaging;

       Stage: manual stage, adjustment range: 125-195mm;

       Fast pressing point Z-axis: Z-axis travel range: 400 mm; Z-axis stepping accuracy: 1.25nm; Repeatability: 5nm; Frequency: 120Hz

       Detector: 4-channel fixed detector: external detector (two-photon): 2 GaAsP (ultrasensitive detector), 2 PMT (photomultiplier tube).

       Scan mode: Galvano-mirror and Resonant-scanner selectable, scanning speed 30fps@512× 512,438fps@512x32 in high-speed scanning mode.

       Analog and digital signal converters for electrophysiology: 6 TTL signal inputs, 6 TTL signal outputs 4-channel analog signal input port, 1-channel analog signal output port;

      Image processing software: FVMPE-RS basic software