Instrument Name:

Olympus SLIDEVIEW VS200  Room: X212

Introduction

The VS200 research slide scanner features a high-speed electric stage, equipped with 6-slide adapters or high-throughput automatic loading (up to 60 slides), high-resolution dual cameras, and a patented shadow correction algorithm. It can quickly scan and stitch images of large tissue slice samples, and is suitable for high-resolution panoramic imaging of various stained samples such as HE, Masson, immunohistochemistry, and immunofluorescence.

Application

Mainly used for scanning samples on slides, such as tissue slices of the brain. Equipped with bright field, dark field, simple polarization, and fluorescence imaging modes, it can quickly achieve batch scanning (up to 60 slides).

Key Technical Specifications

Expected Sample:

Slides (26 mm x 76 mm) with cover slips, thickness: 0.12 mm – 0.17 mm

Fluorescence Channels:

Cy5: Ex. 640/30 nm; Em. 697/60 nm　Cy3: Ex.555/20 nm; Em.595/33 nm　GFP: Ex. 480/30 nm; Em. 519/26 nm　DAPI: Ex. 395/25 nm; Em. 434/32 nm

Detectors:

Color camera and monochrome camera

Objectives:

PLN2×, NA=0.06, WD=5.8mm; UPLFLN4×, NA=0.13, WD=17mm; UPLXAPO10×, NA=0.40, WD=3.1mm (achromatic 400~1000nm); UPLXAPO20×, NA=0.80, WD=0.6mm (achromatic 400~1000nm); UPLXAPO40×, NA=0.95, WD=0.18mm (achromatic 400~1000nm)

Illuminator:

Built-in Köhler illumination, transmitted light, high-intensity high-color-rendering LED (lifespan up to 50,000 hours)

Fluorescence Components:

Fluorescence illuminator with compound lens, electric excitation block turret, electric filter wheel turret, fluorescence light source (FC906)

Scanning Speed:

Bright field: about 80 seconds (20X, 15 mm × 15 mm); Fluorescence: about 3.5 minutes (20X, 15 mm × 15 mm, 4 fluorescence channels, 10 ms exposure each, single layer)

Adapter:

1″ x 3″ slide tray for 10 glass slides, suitable for 26×76 mm slides, max loading 60 slides

Image Processing Software:

Automatic sample detection, barcode reading, focus map, auto scan/stitch, pause/resume, Z-stack, extended focus imaging (EFI), format vsi supporting JPEG/TIFF export, multi-image display, seamless zoom, annotation, screen capture, slide loader control